Sorbonne Université - Faculty of medicine - Saint-Antoine Site
27, rue Chaligny - 75571 Paris cedex 12 - France
Within the Faculty of Medicine of Sorbonne University and the Saint Antoine research center, the B2M "Biomolecules and Molecular Medicine" platform relies on a historical expertise in the field of metabolism and mass spectrometry. Thanks to its proximity to the clinical-biological teams of the Sorbonne University Hospital Group (AP-HP) and in particular the Clinical Metabolomics Department, the platform is positioned on a translational research axis and the transfer of applications to hospital routine and clinical studies.
Thanks to continuous investments, the B2M platform benefits from a fleet of 12 mass spectrometers of the latest generation in terms of sensitivity and resolution. Virtually all analytical approaches are available, whether in GC-MS (gas chromatography coupled to mass spectrometry), LC-MS/MS (liquid chromatography coupled to mass spectrometry), nanoLC, Q-Trap technology and Q-ToF with ion mobility.
Historically, the core of our expertise is focused on the analysis of lipid metabolism with targeted quantitative lipidomic approaches: bile acid profiles, sterols, steroids, fatty acids, reserve lipids, structural lipids, sphingolipids, short chain fatty acids... Recently, we have broadened our fields of analysis, by developing profiles of amino acids and tryptophan derivatives, peptides, and organic acids.
Finally, in addition to analytical problems, mathematical modeling of metabolic pathways and clinical-biological data using "machine learning" approaches completes the know-how of the platform's researchers and engineers.
The B2M platform favors scientific collaborations within the CRSA and SU but remains totally open to other academic and industrial partners. The platform is accredited by the quality assurance organization COFRAC.
The B2M platform is equipped with the following equipment:
• GC-EI/CI-MS (Agilent)
• HPLC-ESI-MS/MS (QqQ) (ABSciex, Shimadzu)
• UPLC-ESI-HRMS (Q-TOF) (Waters)
• NanoLC (Waters)
• Pre-analytical robot (Beckman)
Legend:
1. Waters Synapt G 2S
2. ABSciex Q-TRAP 2000
3. ABSciex Q-TRAP 5500
4. Shimadzu 8060
5. ABSciex Q-TRAP 6500
6. ABSciex Q-TRAP 7500
7. Agilent 5977
Distinct Postprandial Bile Acids Responses to a High-Calorie Diet in Men Volunteers Underscore Metabolically Healthy and Unhealthy Phenotypes. Lamaziere A, Rainteau D, Kc P, Humbert L, Gauliard E, Ichou F, Ponnaiah M, Bouby N, Salem JE, Mallet JM, Guerin M, Lesnik P. Nutrients. 2020 Nov 19;12(11):3545. doi: 10.3390/nu12113545. PMID: 33228154
The Goto-Kakizaki rat is a spontaneous prototypical rodent model of polycystic ovary syndrome. Bourgneuf C, Bailbé D, Lamazière A, Dupont C, Moldes M, Farabos D, Roblot N, Gauthier C, Mathieu d'Argent E, Cohen-Tannoudji J, Monniaux D, Fève B, Movassat J, di Clemente N, Racine C. Nat Commun. 2021 Feb 16;12(1):1064. doi: 10.1038/s41467-021-21308-y. PMID: 33594056
TGR5 controls bile acid composition and gallbladder function to protect the liver from bile acid overload. Bidault-Jourdainne V, Merlen G, Glénisson M, Doignon I, Garcin I, Péan N, Boisgard R, Ursic-Bedoya J, Serino M, Ullmer C, Humbert L, Abdelrafee A, Golse N, Vibert E, Duclos-Vallée JC, Rainteau D, Tordjmann T. JHEP Rep. 2020 Nov 11;3(2):100214. doi: 10.1016/j.jhepr.2020.100214. eCollection 2021 Apr. PMID: 33604531
Direct liquid chromatography tandem mass spectrometry analysis of amino acids in human plasma. Desmons A, Thioulouse E, Hautem JY, Saintier A, Baudin B, Lamazière A, Netter C, Moussa F. J Chromatogr A. 2020 Jul 5;1622:461135. doi: 10.1016/j.chroma.2020.461135. Epub 2020 Apr 17. PMID: 32360058
Fat gain differs by sex and hormonal status in persons living with suppressed HIV switched to raltegravir/etravirine. Assoumou L, Racine C, Fellahi S, Lamaziere A, Farabos D, Beniguel L, Bastard JP, Feve B, Gibowski S, Katlama C, Costagliola D, Capeau J. AIDS. 2020 Oct 1;34(12):1859-1862. doi: 10.1097/QAD.0000000000002644. PMID: 32773470
Changes in circulating miRNA19a-3p precede insulin resistance programmed by intra-uterine growth retardation in mice. Saget S, Cong R, Decourtye L, Endale ML, Martinerie L, Girardet C, Perret C, Clemessy M, Leneuve P, Dinard L, Mohand Oumoussa B, Farabos D, Lamazière A, Lombès M, Moldes M, Fève B, Tregouet D, Le Bouc Y, Kappeler L. Mol Metab. 2020 Dec;42:101083. doi: 10.1016/j.molmet.2020.101083. Epub 2020 Sep 18. PMID: 32956848
Breast-Associated Adipocytes Secretome Induce Fatty Acid Uptake and Invasiveness in Breast Cancer Cells via CD36 Independently of Body Mass Index, Menopausal Status and Mammary Density. Zaoui M, Morel M, Ferrand N, Fellahi S, Bastard JP, Lamazière A, Larsen AK, Béréziat V, Atlan M, Sabbah M. Cancers (Basel). 2019 Dec 13;11(12):2012. doi: 10.3390/cancers11122012. PMID: 31847105
CYP46A1 gene therapy deciphers the role of brain cholesterol metabolism in Huntington's disease. Kacher R, Lamazière A, Heck N, Kappes V, Mounier C, Despres G, Dembitskaya Y, Perrin E, Christaller W, Sasidharan Nair S, Messent V, Cartier N, Vanhoutte P, Venance L, Saudou F, Néri C, Caboche J, Betuing S. Brain. 2019 Aug 1;142(8):2432-2450. doi: 10.1093/brain/awz174. PMID: 31286142
A Placebo-Controlled Trial of Bezafibrate in Primary Biliary Cholangitis. Corpechot C, Chazouillères O, Rousseau A, Le Gruyer A, Habersetzer F, Mathurin P, Goria O, Potier P, Minello A, Silvain C, Abergel A, Debette-Gratien M, Larrey D, Roux O, Bronowicki JP, Boursier J, de Ledinghen V, Heurgue-Berlot A, Nguyen-Khac E, Zoulim F, Ollivier-Hourmand I, Zarski JP, Nkontchou G, Lemoinne S, Humbert L, Rainteau D, Lefèvre G, de Chaisemartin L, Chollet-Martin S, Gaouar F, Admane FH, Simon T, Poupon R. N Engl J Med. 2018 Jun 7;378(23):2171-2181. doi: 10.1056/NEJMoa1714519.
A Liquid Chromatography/Tandem Mass Spectometry Profile of 16 Serum Steroids, Including 21-Deoxycortisol and 21-Deoxycorticosterone, for Management of Congenital Adrenal Hyperplasia. Fiet J, Le Bouc Y, Guéchot J, Hélin N, Maubert MA, Farabos D, Lamazière A. J Endocr Soc. 2017 Feb 10;1(3):186-201. doi: 10.1210/js.2016-1048. eCollection 2017 Mar 1. PMID: 29264476
Partners:
Labels & Certifications:
Contact:
Antonin Lamazière (leader)
Thibaut Eguether (scientific co-responsible)
Aurore Desmons (scientific co-responsible)
Biomolecules and Molecular Medicine Platform (B2M)
Saint Antoine Research Center
Saint Antoine Hospital
184 rue du Faubourg Saint Antoine - 75012 Paris - France
Tel : +33 1 40 01 14 90
INSERM
Kourilsky Building
34 rue Crozatier - 75012 Paris
France
Sorbonne Université Medicine
Saint-Antoine Site
27 rue Chaligny - 75012 Paris
France
