Histomorphology platform of Saint Antoine
Scientific director : Dominique Wendum
Histomorphology platform of Saint Antoine
Scientific director : Dominique Wendum
La plateforme d’HISTOMORPHOLOGIE permet la mise en œuvre de techniques histomorphologiques. Elle met à la disposition de la communauté scientifique du site des matériels, du conseil et de l'assistance. Elle se situe sur le site de Saint Antoine au niveau du bâtiment Pierre Masson, 2ème étage.
La plateforme d'histomorphologie comprend les équipements suivant : acquisition et installation, maintenance, accueil, formation, conseil et assistance aux utilisateurs, interprétation des images, information, communication et animation au profit de la communauté scientifique du site suivi des évolutions technologiques, développements.
• Histopathology (inclusion, microtome section and staining)
The microtome is a device which allows, with the help of a razor, to make thin sections, about 4 to 5 microns, from a block obtained after inclusion in kerosene. These sections, after spreading, are used either for staining or for an Immuno-Histochemistry (IHC) technique or microdissection. Thicker sections can also be made and used to extract DNA
Reservation: per hour
• Cryomicrotomy
The cryostat is a device allowing to make thin sections with a razor from frozen samples. These sections, after spreading, are used either for staining, or for an Immuno-Histochemistry (IHC) technique or a microdissection. Thicker sections can also be made and used to extract DNA or RNA.
Reservation: per hour
• Immunohistochemistry
Dewaxing trays (xylene and ethanol); Pre-treatment buffers: citrate (pH6.0), EDTA (pH8.0 and pH9.0); Boxes for hand IHC; Cones for propipettes; Glassware; AEC; Hemalun; Slides mounting kit; Toxic waste canisters; Gloves; Bench top cleaner and paper towels.
Reservation: per day
• Laser microdissection
Laser microdissection allows to isolate and collect cells within tissues according to their phenotypic and functional characteristics. A DNA, RNA, proteomic study can be done after selecting the cells.
Reservation: per 1/2 day
• Microarray tissue
It consists in building a kerosene block using the principle of coring from a collection of donor blocks. This method allows to study simultaneously on a single histological section a large number of samples. Time and material saving is one of the major advantages of this technique.
Reservation: per 1/2 day
• Morphométry EXPLORA NOVA
Reservation: per 1/2 day
INSERM
Kourilsky Building
34 rue Crozatier - 75012 Paris
France
Sorbonne Université Medicine
Saint-Antoine Site
27 rue Chaligny - 75012 Paris
France
