• Wednesday, 08 July 2020 - 01:23:22

Metabolism and age-related joint diseases

Francis Berenbaum
Team Leader : Francis Berenbaum
Administrative contact : Olguine Trismercin

Hôpital Saint-Antoine - Bâtiment Kourilsky - 5ème étage - 184, rue du Faubourg Saint-Antoine, 75012 Paris, France


 La Rhumatologie à Saint-Antoine : LaRhumato.fr

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Research metabolic diseases and age-related joint pathologies

Osteoarthritis (OA), a degenerative disease primarily affecting diarthrodial joints, afflicts 6M people in France, with a global prevalence of disease recently estimated to exceed 250M. Overall, diarthrodial/spinal OA is the most prevalent cause of disability, surpassing the next four medical disorders combined, with annual medical expenditures exceeding 3.4 billion €, a doubling in 10 years. Unfortunately, the only clinically accepted treatment strategies are palliative, with no disease modifying OA drug approved for use in humans. Thus an effective treatment for OA is a critical unmet need


Our team associates physiologists, cell biologists, biochemists, rheumatologists and orthopedic surgeons devoted to find new therapeutic targets in osteoarthritis (OA). For this objective, we take advantage of the recent knowledge about the pathophysiology of the disease:

  • OA has initially been considered as cartilage-driven only. We now know that OA is a much more complex disease, involving not only cartilage, but also bone and synovium interacting altogether. Indeed, studies suggest that OA structural progression may result from altered communication between subchondral bone cells (e.g., osteoblasts, osteocytes, endothelial cells, adipocyte), chondrocytes and synoviocytes.
  • Because obesity and cardiometabolic diseases are risk factors for OA not only in weight-bearing joints but also in the hands, systemic mediators and systemic low-grade inflammation are suggested to play a critical role in initiatiting and/or aggravating OA.

Based on these recent discoveries, our main projects currently under investigation are:

  1. Finding novel mediators involved in the disrupted communication between cartilage and the subchondral bone that could be therapeutic targets.
  2. Determining the role of inflammatory/metabolic/neuronal external cues on inter-tissular miscommunications that could be manipulated for therapeutic purposes.

Methodologies and tools used:

  • Primary culture of articular chondrocytes (mouse, human) and costal chondrocytes (mouse), of osteoblasts (human and murine 3D membranes), of human synoviocytes and of mesenchymal stem cells (from human and rat bone marrow and adipose tissues)
  • Control of chondrocyte phenotype (hypertrophic differentiation and fibroblastic dedifferentiation)
  • Bone/cartilage communication model (murine)
  • Differentiation of mesenchymal stem cells into chondrocytes, adipocytes and osteoblasts
  • Cell assay (proliferation, adhesion, angiogenesis)
  • Cellular analysis (Western-blot, proteolytic activity, ELISA, qRT-PCR, ARN interference, immunocytology)
  • BioJoint biobank of human OA tissues for tissue, cell, biochemical and molecular analyses
  • Tissue analysis (histology, immunohistochemistry)
  • Experimental models for Osteoarthritis (Mouse)

SUMMARY OF SIGNIFICANT EVENTS

1- Finding novel mediators involved in the disrupted communication between cartilage and the subchondral bone that could be therapeutic targets.

Osteoarthritis (OA) is characterized by a pathological remodeling of the osteochondral junction, which is pivotal in cartilage disappearance and subchondral bone sclerosis. This remodeling can be explained by a pathological reactivation of the endochondral ossification process, including a hypertrophic differentiation of chondrocytes, a mineralization of the cartilage matrix, and enhanced angiogenesis and a resorption of bone followed by new synthesis. The project investigates cell and molecular determinants involved in OA osteochondral bone remodeling. On one hand, we aim at determining factors acting on chondrocyte hypertrophic differentiation (collaborative project granted by SFR) and those produced by hypertrophic chondrocytes, which can impact both angiogenesis and osteoclastogenesis (I. Toillon’s PhD granted by ARDoC program). On the other hand, we are studying the consequences of an excessive mechanical load on the angiogenic activity of osteoblasts. We are also investigating the role of bone marrow adipose tissue (adipocytes and mesenchymal stromal cells) (grant from the SFR Network program and N. Zapata’s postdoc granted by FRM). Our published and unpublished data indeed suggest that intra-articular adipose tissues are new tissue players involved in OA (grant from Ramsay Générale de Santé). We are also developing in collaboration with a chemist (Dr. J. Landousli) a new approach based on atomic force microscopy in order to analyze the structural modifications of the osteochondral junction in OA (grant from IPV – Sorbonne Université program for the PhD of I. Jaabar).

Role of 14-3-3: Recent studies have shown the implication of the innate immune system and low-grade inflammation in OA progression. Our team identified 14-3-3ε as a new catabolic factor, produced by the subchondral bone and able to induce a catabolic phenotype on chondrocytes. We discovered that these effects are mediated, at least in part, by TLR2 or TLR4 signalling, leading to the activation of innate immunity as others alarmins. Moreover, innate alarmins can stimulate the activation of resident immune cells of the synovium, particularly macrophages leading to synovitis. We found that polarization of macrophages stimulated by recombinant 14-3-3ε seems to polarize them toward a M1 inflammatory phenotype. Taken together, our results designate 14-3-3ε as a novel alarmin in OA, triggering catabolic and inflammatory effects through TLRs signaling. This new alarmin candidate could represent a new target either for therapeutic and/or prognostic purposes.

  1. S. Priam, C. Bougault, X. Houard, C. Salvat, M. Gosset, F. Berenbaum and C. Jacques. Identification of soluble 14-3-3 as a novel subchondral bone mediator involved in cartilage degradation in osteoarthritis. Arthritis and Rheumatism. 2013 Jul;65(7):1831-42.
  2. Nefla M, Sudre L, Denat G, Priam S, Andre-Leroux G, Berenbaum F, Jacques C. The pro-inflammatory cytokine s14-3-3ε is a ligand of CD13/Aminopeptidase N in cartilage. J Cell Sci. 2015 Sep 1;128(17):3250-62.
  3. Nefla M, Holzinger D, Berenbaum F and Jacques C. The danger from within: alarmins in arthritis. Nat Rev Rheumatol. 2016 12(11):669- 683.
  4. Eymard F, Pigenet A, Citadelle D, Tordjman J, Foucher L, Rose C, Flouzat Lachaniette CH, Clément K, Berenbaum F, Chevalier X, Houard X. Knee and hip intra-articular adipose tissues (IAATs): a common phenotype for a central player in osteoarthritis. Ann. Rheum Dis. 2017;76(6):1142-48.
  5. Eymard F, Pigenet A, Citadelle D, Flouzat-Lachaniette CH, Poignard A, Benelli C, Berenbaum F, Chevalier X, Houard X. Infrapatellar fat pad induces an inflammatory and pro-degradative phenotype on autologous fibroblast-like synoviocytes from severe knee osteoarthritis patients. Arthritis Rheumatol. 2014; 66(8):2165-74.

2- Determining the role of inflammatory/metabolic/neuronal external cues on inter-tissular miscommunications that could be manipulated for therapeutic purposes.

Metabolic OA phenotype (1): Considering the association between metabolic diseases and OA (2-5), we aimed to decipher why OA is more frequent and more severe in patients with type 2 diabetes mellitus, participating thus to the metabolic diseases-associated OA phenotype. Using cartilage explants from diabetic and non-diabetic patients, we demonstrated that the anti-oxidant axis Nrf2/HO-1 is defective. This phenomenon is also observed in cultured murine chondrocytes subjected to high or normal glucose exposure. The impairment of Nrf2/HO-1 is responsible for a more pronounced inflammatory phenotype of chondrocytes, elevating thus the level of chondrocytic inflammation.

Ageing-related OA phenotype (6): Accumulation of advanced glycation end-products (AGEs) during tissue ageing is part of the ageing-related OA phenotype by modifying the biomechanical properties of cartilage and by inducing chondrocyte activation. We have found an age-dependent accumulation of AGEs, notably CML (Nε-carboxymethyl-lysine) in OA cartilage. Such a phenomenon could be due to a chondrocytic impairment of the adaptative mechanism of Glo-1, a detoxifying enzyme that eliminates glyoxal, the precursor of CML. This impairment of Glo-1 enzymatic activity could be due to IL-1-related inflammation.

Based on an integrative view of OA pathophysiology. we are investigating the relationship between autonomic nervous system (and especially its main mediator acetylcholine) and OA. Taking advantage of a recent sophisticated method that uses a whole joint immunolabeling protocol (after 3Disco clearing, in toto 3D immunofluorescence), we are currently studying the cholinergic innervation of joint tissues (i.e., subchondral bone and synovium) in human and murine joint samples (collaboration with Alain CHEDOTAL Institut de la Vision, INSERM, Paris, France)7. We are investigating whether chondrocytes and osteoblasts are able to produce themselves acetylcholine (i.e., non-neuronal production of acetylcholine) and whether the acetylcholine/acetylcholine receptors axis (nicotinic alpha-7 receptor (Chrna7) may regulate joint inflammation (collaboration with Uwe MASKOS, Unité de neurobiologie intégrative des systèmes cholinergiques, CNRS UMR 3571, Institut Pasteur, Paris, France) (PhD thesis of Dr Alice COURTIES, granted by AP-HP and French Society of Rheumatology).

  1. Courties A, Berenbaum F, Sellam J. The Phenotypic Approach to Osteoarthritis: A Look at Metabolic Syndrome-Associated Osteoarthritis. Joint Bone Spine. 2018 Dec 22. [Epub ahead of print]
  2. Louati K, Vidal C, Berenbaum F, Sellam J. Association between diabetes mellitus and osteoarthritis: systematic literature review and meta-analysis. RMD Open. 2015 Jun 2;1(1):e000077.
  3. Vaamonde-Garcia C, Courties A, Pigenet A, Laiguillon MC, Sautet A, Houard X,Kerdine-Römer S, Meijide R, Berenbaum F, Sellam J. The nuclear factor-erythroid 2-related factor/heme oxygenase-1 axis is critical for the inflammatory features of type 2 diabetes-associated osteoarthritis. J Biol Chem. 2017 Sep 1;292(35):14505-14515
  4. Courties A, Sellam J, Berenbaum F. Metabolic syndrome-associated osteoarthritis. Curr Opin Rheumatol. 2017 Mar;29(2):214-222.
  5. Tomi AL, Sellam J, Lacombe K, Fellahi S, Sebire M, Rey-Jouvin C, Miquel A,Bastard JP, Maheu E, Haugen IK, Felson DT, Capeau J, Girard PM, Berenbaum F,Meynard JL. Increased prevalence and severity of radiographic hand osteoarthritis in patients with HIV-1 infection associated with metabolic syndrome: data from the cross-sectional METAFIB-OA study. Ann Rheum Dis. 2016 Dec;75(12):2101-2107
  6. Trellu S, Courties 1, Jaisson S, Gorisse L, Gillery P, Kerdine-Römer S, Vaamonde-Garcia C, Houard X, Ehkirch FP, Sautet A, Friguet B, Jacques C, Berenbaum F, Sellam J. Impairment of glyoxalase-1, an advanced glycation end-products detoxifying enzyme, induced by inflammation in aged-related osteoarthritis, Arthritis Research & Therapy, in press, 2019
  7. Courties A, Sellam J, Berenbaum F. Role of the autonomic nervous system in osteoarthritis. Best Pract Res Clin Rheumatol. 2017 Oct;31(5):661-675

 

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